Large scale multi-residue methods by gas and liquid chromatography-mass spectrometry for the determination of pesticide residues in food matrices
Botitsi, E., Antoniou, S., Tsipi, D.
Abstract:
Nowadays, more than 1600 pesticides belonging to more than 100 chemical classes are in use worldwide for food production. In the EU, Regulation (EC) 396/2005 sets maximum pesticide residue limits in food (MRLs), the majority of which ranges over the concentration range 0.01-1 mg / kg. Large scale multi-residue methods based on general extraction procedures in combination with hyphenated instrumental analysis techniques such as gas and liquid chromatography with mass spectrometry are currently used in analytical laboratories working in the field of Pesticide Residues Analysis. In our laboratory, a multi- residue method based on QuEChERS method in combination with LC-MS/MS and GC-MS/MS techniques has been developed for the detection and quantification of pesticide residues, parent compounds and their metabolites, in various food matrices, fulfilling the performance criteria established in the European Union guidelines (SANTE Doc. No 11945/2015). A LC-MS/MS method using QqQ-MS technology in the SRM acquisition mode has been developed for the analysis of more than 250 compounds. Additionally, a GC-MS/MS method using Ion-Trap MSn technology has been applied for the determination of more than 80 GC-amenable compounds. More than 2000 pesticide/food commodity combinations have been validated using the LC- and GC-MS/MS methods, achieving recoveries in the range 70-120% with reproducibility values in terms of relative standard deviations less than 20%; limits of quantification of 0.01mg/Kg were established in most of the cases. Both methods have been successfully applied for the monitoring of multiclass pesticide residues in food samples in the frame of National and EU - Multi Annual Coordinated Control Programs.
Keywords:
pesticides, LC-MS/MS, GC-MSn
Download:
IMEKO-TC23-2017-014.pdf
DOI:
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Event details
IMEKO TC:
TC23
Event name:
3rd IMEKOFOODS Conference
Title:

Metrology Promoting Standardization and Harmonization in Food and Nutrition

Place:
Thessaloniki, GREECE
Time:
01 October 2017 - 04 October 2017
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